Usage Guide

Research peptides arrive as a sterile lyophilized (freeze-dried) powder inside a sealed vial. Before use in a study, the powder is reconstituted with bacteriostatic water (BAC water) to produce a working solution at a known concentration. From that solution, measured volumes are drawn into a U-100 insulin syringe for each dose.

The three numbers that matter are the vial mass (mg of peptide), the diluent volume (mL of BAC water), and the target dose. Our Dosage Calculator turns those inputs into a concentration, a draw volume, and the exact mark on a U-100 syringe.

• Sealed vial of lyophilized peptide.
• Bacteriostatic water (0.9% benzyl alcohol) — 1 mL to 10 mL, depending on protocol.
• U-100 insulin syringe (100 units = 1 mL), 0.3 mL or 0.5 mL barrel.
• Alcohol swabs for the vial septum.
• Sharps container for safe disposal.
• A refrigerator at 2–8 °C for storing the reconstituted vial.

1. 1

   Bring both vials to room temperature for 10–15 minutes. Cold glass can crack and cold solutions sting.

2. 2

   Swab the rubber septum of the peptide vial and the BAC water vial with alcohol and let them dry.

3. 3

   Draw the planned volume of BAC water (e.g. 2 mL) into a clean syringe.

4. 4

   Insert the needle into the peptide vial at an angle and release the water slowly down the inside wall of the glass — never aim the stream directly onto the powder.

5. 5

   Withdraw the syringe. Swirl the vial gently to dissolve the powder. Do NOT shake — agitation can denature the peptide.

6. 6

   Wait 30–60 seconds. The solution should be clear and free of particles. Record the reconstitution date and BAC water volume on the label.

7. 7

   Store the reconstituted vial in the refrigerator at 2–8 °C between draws.

The amount of BAC water you add changes the concentration of every subsequent draw — it does not change the total mass of peptide in the vial. More water means a larger draw volume per dose (easier to read on the syringe) but a more dilute solution.

Numbers shown for a 10 mg vial with a 0.25 mg target dose. Use the calculator for any other combination.

A U-100 insulin syringe is calibrated so that 100 units = 1 mL. Each small mark on a 1 mL barrel is therefore 0.01 mL. Volumes below 0.05 mL (5 units) are difficult to read accurately — if your protocol calls for a smaller draw, increase the BAC water volume so the dose lands in a more readable range.

• Unopened lyophilized vial: store at -20 °C, protected from light. Most peptides remain stable for 24+ months.
• Reconstituted vial: refrigerate at 2–8 °C. Aim to use within 14–30 days depending on the compound.
• Do not freeze a reconstituted vial. Freeze-thaw cycles cause aggregation and loss of activity.
• Keep away from direct light and avoid leaving the vial at room temperature for extended periods.
• Always refer to the storage note on the individual product page for compound-specific guidance.

• Work on a clean, uncluttered bench. Wash hands and wear gloves.
• Swab the rubber septum with alcohol before every entry.
• Use a fresh sterile needle for every draw — never re-use needles.
• Dispose of used syringes in a designated sharps container.
• Label every vial with compound, concentration, BAC water volume, and reconstitution date.